CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation
Figure 1
Treg gating strategy. Representative bivariate dot plots illustrating gating strategy used to enumerate lymphocytes with the CD3+CD4+CD25highCD127low phenotype (Treg) in the same blood sample. The settings showed here were used for the analysis of all peripheral blood samples. Events corresponding to Treg are shown as black dots, events corresponding to other lymphocytes are shown as gray dots in all bivariate dot plots. (a) Gate shows CD3+CD4+ events corresponding to T-helper lymphocytes; (b) CD25highCD127low events corresponding to Treg are displayed in the gate; (c) bivariate dot plot illustrating the CD4+CD25high phenotype pattern of Treg; (d) bivariate dot plot illustrating the CD4+CD127low phenotype pattern of Treg: (e) and (f) bivariate dot plots showing appropriate isotype controls. CD: cluster of differentiation.
