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The Scientific World Journal
Volume 2012 (2012), Article ID 647352, 10 pages
http://dx.doi.org/10.1100/2012/647352
Research Article

The Chemical Composition of Endotoxin Isolated from Intestinal Strain of Desulfovibrio desulfuricans

1Department of Biochemistry, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, Poland
2Department of Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, Poland
3Department of Instrumental Analysis, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, Poland

Received 14 October 2011; Accepted 27 December 2011

Academic Editor: Raúl Rivas

Copyright © 2012 Jolanta Lodowska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Desulfovibrio desulfuricans anaerobes are constituents of human alimentary tract microflora. There are suggestions that they take part in the pathogenesis of periodontitis and some gastrointestinal inflammatory disorders, such as ulcerative colitis or Crohn’s disease. Endotoxin is one of Gram-negative bacteria cellular components that influence these microorganisms pathogenicity. Endotoxin is a lipid-polisaccharide heteropolymer consisting of three elements: lipid A, core oligosaccharide, and O-specific polysaccharide, also called antigen-O. The biological activity of lipopolysaccharide (LPS) is determined by its structure. In this study, we show that rhamnose, fucose, mannose, glucose, galactose, heptose, and 2-keto-3-deoxyoctulosonic acid (Kdo) are constituents of D. desulfuricans endotoxin oligosaccharide core and O-antigen. Lipid A of these bacteria LPS is composed of glucosamine disaccharide substituted by 3-acyloxyacyl residues: ester-bound 3-(dodecanoyloxy)tetradecanoic, 3-(hexadecanoyloxy)tetradecanoic acid, and amide-bound 3-(tetradecanoyloxy)tetradecanoic acid.