Research Article
Phenotypic Detection of Metallo-β-Lactamase in Imipenem-Resistant Pseudomonas aeruginosa
Table 1
Detection of MBL activity and MBL genes of IRPA clinical isolates from Malaysia.
| | Bacterial strain | CDT | DDST | IP/IPI E-test |
PCR | | IMP (mm) | IMP + EDTA (mm) | MBL index | MBL index | IP (μg/mL) | IPI (μg/mL) | MBL index |
| | Ps1 | 10 | 22 | 12 | + | + | 256 | 1 | 256 | + | IMP-7 | | Ps2 | 12 | 30 | 18 | + | + | 256 | 4 | 64 | + | IMP-4 | | Ps3 | 10 | 24 | 14 | + | + | 256 | 1 | 256 | + | IMP-4 | | Ps4 | 10 | 22 | 12 | + | + | 192 | <1 | >192 | + | VIM-2 | | Ps5 | 10 | 20 | 10 | + | + | 192 | 1 | 192 | + | VIM-2 | | Ps6 | 10 | 24 | 14 | + | + | 192 | <1 | >192 | + | VIM-2 | | Ps7 | 10 | 25 | 15 | + | + | 192 | <1 | >192 | + | VIM-2 | | Ps8 | 10 | 20 | 10 | + | + | 192 | <1 | >192 | + | VIM-2 | | Ps9 | 11 | 21 | 10 | + | + | 192 | <1 | >192 | + | VIM-2 | | Ps10 | 11 | 26 | 15 | + | + | 192 | 1 | 192 | + | VIM-2 | | Ps11 | 10 | 24 | 14 | + | + | 192 | <1 | >192 | + | VIM-2 | | Ps12 | 12 | 21 | 9 | + | + | 192 | 2 | 96 | + | VIM-2 | | Ps13 | 12 | 22 | 10 | + | + | 192 | 2 | 96 | + | VIM-2 | | Ps14 | 10 | 22 | 12 | + | + | 192 | <1 | >192 | + | IMP-7 | | Ps15 | 12 | 30 | 18 | + | + | 192 | 4 | 48 | + | IMP-7 | | Ps16 | 10 | 23 | 13 | + | + | 192 | 2 | 96 | + | IMP-7 | | Ps17 | 10 | 23 | 13 | + | + | 192 | 2 | 96 | + | IMP-7 | | Ps18 | 10 | 27 | 17 | + | + | 192 | 2 | 96 | + | IMP-7 | | Ps19 | 10 | 17 | 7 | + | + | 192 | 2 | 96 | + | IMP-7 | | Ps20 | 10 | 23 | 13 | + | + | 128 | 2 | 64 | + | VIM-2 | | Ps21 | 10 | 25 | 15 | + | + | 128 | <1 | >128 | + | VIM-2 | | Ps22 | 10 | 29 | 19 | + | + | 128 | <1 | >128 | + | VIM-2 | | Ps23 | 10 | 27 | 17 | + | + | 128 | 1 | 128 | + | VIM-11 | | Ps24 | 10 | 20 | 10 | + | + | 128 | <1 | >128 | + | VIM-2 | | Ps25 | 10 | 33 | 23 | + | + | 128 | 2 | 64 | + | VIM-2 | | Ps26 | 10 | 27 | 17 | + | + | 128 | 2 | 64 | + | VIM-2 | | Ps27 | 11 | 21 | 10 | + | + | 128 | 2 | 64 | + | VIM-2 | | Ps28 | 12 | 30 | 18 | + | + | 128 | 1 | 128 | + | IMP-7 | | Ps29 | 12 | 21 | 9 | + | + | 128 | 1 | 128 | + | IMP-7 | | Ps30 | 11 | 21 | 10 | + | + | 128 | 1 | 128 | + | IMP-7 | | Ps31 | 10 | 23 | 13 | + | + | 128 | 1 | 128 | + | IMP-7 | | Ps32 | 11 | 22 | 11 | + | + | 96 | <1 | >96 | + | IMP-7 | | Ps33 | 10 | 22 | 12 | + | − | 32 | 6 | 5 | − | − | | Ps34 | 12 | 18 | 6 | − | − | 24 | 4 | 6 | − | − | | Ps35 | 10 | 27 | 17 | + | − | 24 | 4 | 6 | − | − | | Ps36 | 10 | 25 | 15 | + | − | 24 | 4 | 6 | − | − | | Ps37 | 12 | 23 | 11 | + | − | 24 | 4 | 6 | − | − | | Ps38 | 13 | 25 | 12 | + | − | 24 | 4 | 6 | − | − | | Ps39 | 10 | 19 | 9 | + | − | 24 | 4 | 6 | − | − | | Ps40 | 10 | 18 | 5 | + | − | 24 | 4 | 6 | − | − | | Ps41 | 10 | 25 | 15 | + | − | 24 | 4 | 6 | − | − | | Ps42 | 12 | 18 | 6 | − | − | 24 | 4 | 6 | − | − | | Ps43 | 10 | 20 | 10 | + | − | 24 | 4 | 6 | − | − | | Ps44 | 10 | 25 | 15 | + | + | 24 | 4 | 6 | − | − | | Ps45 | 9 | 30 | 21 | + | − | 24 | 4 | 6 | − | − | | Ps46 | 10 | 24 | 14 | + | − | 24 | 4 | 6 | − | − | | Ps47 | 10 | 16 | 6 | − | − | 24 | 4 | 6 | − | − | | Ps48 | 12 | 24 | 12 | + | − | 24 | 4 | 6 | − | − | | Ps49 | 13 | 20 | 7 | + | − | 24 | 4 | 6 | − | − | | Ps50 | 12 | 18 | 6 | − | − | 16 | 2 | 8 | + | − | | Ps51 | 10 | 20 | 10 | + | + | 16 | 2 | 8 | + | − | | Ps52 | 12 | 18 | 6 | − | − | 16 | 3 | 5 | − | − | | Ps53 | 11 | 21 | 10 | + | − | 16 | 3 | 5 | − | − | | Ps54 | 10 | 23 | 13 | + | − | 16 | 3 | 5 | − | − | | Ps55 | 13 | 25 | 12 | + | − | 16 | 4 | 4 | − | − | | Ps56 | 11 | 24 | 13 | + | − | 16 | 3 | 5 | − | − | | Ps57 | 11 | 21 | 10 | + | − | 16 | 4 | 4 | − | − | | Ps58 | 10 | 27 | 17 | + | − | 16 | 4 | 4 | − | − | | Ps59 | 12 | 23 | 11 | + | − | 16 | 3 | 5 | − | − | | Ps60 | 10 | 16 | 6 | − | − | 16 | 3 | 5 | − | − | | Ps61 | 10 | 22 | 12 | + | − | 16 | 4 | 4 | − | − | | Ps62 | 10 | 32 | 22 | + | − | 16 | 3 | 5 | − | − | | Ps63 | 10 | 22 | 12 | + | − | 16 | 4 | 4 | − | − | | Ps64 | 10 | 20 | 10 | + | − | 16 | 4 | 4 | − | − | | Ps65 | 11 | 21 | 10 | + | − | 12 | 2 | 6 | − | − | | Ps66 | 14 | 20 | 6 | − | − | 12 | 2 | 6 | − | − | | Ps67 | 13 | 18 | 5 | − | − | 12 | 2 | 6 | − | − | | Ps68 | 10 | 13 | 3 | − | − | 12 | 3 | 4 | − | − | | Ps69 | 12 | 14 | 2 | − | − | 12 | 4 | 3 | − | − | | Ps70 | 10 | 27 | 17 | + | − | 12 | 2 | 6 | − | − | | Ps71 | 10 | 20 | 10 | + | − | 8 | <1 | >8 | + | − | | Ps72 | 12 | 21 | 9 | + | − | 8 | <1 | >8 | + | − | | Ps73 | 12 | 21 | 9 | + | − | 8 | <1 | >8 | + | − | | Ps74 | 10 | 24 | 14 | + | − | 8 | <1 | >8 | + | − | | Ps75 | 10 | 14 | 4 | − | − | 8 | <1 | >8 | + | − | | Ps76 | 12 | 30 | 18 | + | − | 8 | <1 | >8 | + | − | | Ps77 | 12 | 18 | 6 | − | − | 8 | <1 | >8 | + | − | | Ps78 | 12 | 18 | 6 | − | − | 8 | <1 | >8 | + | − | | Ps79 | 10 | 16 | 6 | − | − | 8 | 1 | 8 | + | − | | Ps80 | 11 | 17 | 6 | − | − | 8 | 1 | 8 | + | − | | Ps81 | 12 | 18 | 6 | − | − | 8 | <1 | >8 | + | − | | Ps82 | 12 | 18 | 6 | − | − | 8 | <1 | >8 | + | − | | Ps83 | 14 | 18 | 4 | − | − | 8 | <1 | >8 | + | − | | Ps84 | 13 | 17 | 4 | − | − | 8 | <1 | >8 | + | − | | Ps85 | 13 | 19 | 6 | − | − | 8 | <1 | >8 | + | − | | Ps86 | 12 | 18 | 6 | − | − | 8 | 1 | 8 | + | − | | Ps87 | 12 | 18 | 6 | − | − | 8 | 1 | 8 | + | − | | Ps88 | 10 | 14 | 4 | − | − | 8 | <1 | >8 | + | − | | Ps89 | 11 | 15 | 4 | − | − | 8 | <1 | >8 | + | − | | Ps90 | 12 | 17 | 5 | − | − | 8 | <1 | >8 | + | − |
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