The Effect of High Concentrations of Glufosinate Ammonium on the Yield Components of Transgenic Spring Wheat (Triticum aestivum L.) Constitutively Expressing the bar Gene
Figure 2
Detection of RNA transcripts derived from the herbicide resistance gene bar by electrophoresis of RT-PCR products. The white arrow shows the expected 375 bp fragment. Markers: λ-DNA digested with EcoRI and HindIII restriction enzymes. Samples from left to right: 1–14: according to increasing herbicide concentrations (1 refers to 0 while 14 refers to 5000 mg · L−1 of glufosinate ammonium), 15: nontransgenic CY-45 plant, 16: distilled water, 17: pAHC25 plasmid DNA.
