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The Scientific World Journal
Volume 2012, Article ID 680108, 8 pages
Research Article

Preparation and Characterization of Alginate and Psyllium Beads Containing Lactobacillus acidophilus

1Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran
2Gastrointestinal and Liver Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
4Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Received 31 October 2011; Accepted 14 December 2011

Academic Editors: J. Ali, A. Concheiro, and P. Danckwerts

Copyright © 2012 Farzaneh Lotfipour et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper describes preparation and characterization of beads of alginate and psyllium containing probiotic bacteria of Lactobacillus acidophilus DMSZ20079. Twelve different formulations containing alginate (ALG) and alginate-psyllium (ALG-PSL) were prepared using extrusion technique. The prepared beads were characterized in terms of size, morphology and surface properties, encapsulation efficiency, viabilities in acid (pH 1.8, 2 hours) and bile (0.5% w/v, 2 hours) conditions, and release in simulated colon pH conditions. The results showed that spherical beads with narrow size distribution ranging from 1.59±0.04 to 1.67±0.09 mm for ALG and from 1.61±0.06 to 1.80±0.07 mm for ALG-PSL with encapsulation efficiency higher than 98% were achieved. Furthermore, addition of PSL into ALG enhanced the integrity of prepared beads in comparison with ALG formulations. The results indicated that incorporation of PSL into alginate beads improved viability of the bacteria in acidic conditions as well as bile conditions. Also, stimulating effect of PSL on the probiotic bacteria was observed through 20-hour incubation in simulated colonic pH solution. According to our in vitro studies, PSL can be a suitable polymer candidate for partial substitution with ALG for probiotic coating.