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The Scientific World Journal
Volume 2012, Article ID 685028, 6 pages
Research Article

Virulence Genes and Antimicrobial Resistance Profiles of Pasteurella multocida Strains Isolated from Rabbits in Brazil

1Programa de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil
2Laboratório de Sanidade Suína e Virologia, VPS/FMVZ/USP, Cidade Universitária, Avenida Prof. Dr. Orlando Marques de Paiva 87, CEP 05508 270 São Paulo, SP, Brazil

Received 22 May 2012; Accepted 29 June 2012

Academic Editors: M. S. Cupp and D. Endoh

Copyright © 2012 Thais Sebastiana Porfida Ferreira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Pasteurella multocida is responsible for a wide range of diseases in domestic animals. In rabbits, the agent is related to nasal discharge, pneumonia, otitis media, pyometra, orchitis, abscess, and septicemia. One hundred and forty rabbits with respiratory diseases from four rabbitries in São Paulo State, Brazil were evaluated for the detection of P. multocida in their nasal cavities. A total of twenty-nine animals were positive to P. multocida isolation, and 46 strains were selected and characterized by means of biochemical tests and PCR. P. multocida strains were tested for capsular type, virulence genes, and resistance profile. A total of 45.6% (21/46) of isolates belonged to capsular type A, and 54.34% (25/46) of the isolates were untypeable. None of the strains harboured toxA or pfhA genes. The frequency of the other twenty genes tested was variable, and the data generated was used to build a dendrogram, showing the relatedness of strains, which were clustered according to origin. Resistance revealed to be more common against sulfonamides and cotrimoxazole, followed by erythromycin, penicillin, and amoxicillin.