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The Scientific World Journal
Volume 2012, Article ID 691569, 5 pages
Research Article

Use of Enzymatic Bio-Fenton as a New Approach in Decolorization of Malachite Green

1Department of Chemical Engineering, University of Tabriz, Tabriz 51666-17615, Iran
2Department of Applied Chemistry, University of Tabriz, Tabriz 51666-17615, Iran

Received 30 October 2011; Accepted 12 January 2012

Academic Editors: J. Canário, G.-C. Fang, and P. J. Lasier

Copyright © 2012 Afzal Karimi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An enzymatic reaction using glucose oxidase was applied for in situ production of hydrogen peroxide for use in simultaneously Fenton's reaction in decolorization of malachite green. It was found that decolorization rate increased by increasing of glucose concentration from 0.2 g/L to 1.5 g/L. Decolorization rate showed different behaviors versus temperature changes. Initial rate of decolorization process was increased by increasing of temperature; after 30 minutes, especially at temperatures above 30°C, the decolorization rate was gradually reduced. The pH value in the reaction media was decreased from natural to about p H = 3 which had synergic effect on the Fenton process by stabilizing of Fe2+ ions.