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| Target | Forward primer | Reverse primer |
|
| β-Catenin | 5′-AGCGTGGACAATGGCTACTCAA-3′ | 5′-AAACATAGCAGCTCGTACCCTCT-3′ |
| APC | 5′-AGGACATG TTCTATGCCT TATGCC-3′ | 5′-CTTGGCATTAGATGAAGGTGTGGA-3′ |
| GSK-3β | 5′-ATCGGGATATTAAACCGCAGAACC-3′ | 5′-CTGTGTAGTTTGGGTTCATTTCTCT-3′ |
| TCF1 | 5′-CCTCTCTGGCTTCTACTCCCT-3′ | 5′-CAGCCTGGGTATAGCTGCATGT-3′ |
| TCF4 | 5′-AGGCACAGCTGTTTGGTCTAGAA-3′ | 5′-TCTCAGGGCCACGCCATCTTCA-3′ |
| APC segment 1 codons 2–812 | 5′-GGATCCTAATACGACTCACTATAGGAACAGACCACCATG GCTGCAGCTTCATATGATC-3′ | 5′-CTGACCTATTATCATCATGTCG-3′ |
| APC segment 2 codons 654–1225 | 5′-GGATCCTAATACGACTCACTATAGGAACAGACCACCATG CAAATCCTAAGAGAGAACAACT-3′ | 5′-GAGGATC CATTAGATGAAGGTGTGGACG-3′ |
| APC segment 3 codons 1050–1693 | 5′-GGATCCTAATACGACTCACTATAGGAACAGACCACCATG GCAAGACCCAAACACATAATAG-3′ | 5′-GAGGATC CTGTAGGAATGGTATCTCG-3′ |
| APC segment 4 codons 1582–2337 | 5′-GGATCCTAATACGACTCACTATAGGAACAGACCACCATG GCCATGCCAACAAAGTCATCA-3′ | 5′-CTTATTCCATTTCTACCAGGGGAA-3′ |
| APC segment 5 codons 2123–2698 | 5′-GGATCCTAATACGACTCACTATAGGAACAGACCACCATGGGTTTATCTAGACAAGCTTCG-3′ | 5′-TTGAATCTTTAATGTTTGGATTTGC-3′ |
| APC β-catenin binding region | 5′-AGGACATGTTCTATGCCTTATGCC-3′ | 5′-CTTGGCATTAGATGAAGGTGTGGA-3′ |
| APC mutational hot spot at codon 1309 | 5′-AACGTCATGTGGATCAGCCTATTG-3′ | 5′-GCTGGCAATCGAACGACTCTCAA-3′ |
| APC mutational cluster region | 5′-TTTAGCAGATGTACTTCTGTCAGTT-3′ | 5′-ATAGGTCCTTTTCAGAATCAATAGT-3′ |
| β-catenin exon3 | 5′-CCAATCTACTAATGCTAATACTG-3′ | 5′-CTGCATTCTGACTTTCAGTAAGG-3′ |
| GAPDH | 5′-GGTGAAGGTCGGAGTCAACGGA-3′ | 5′-GAGGGATCTCGCTCCTGGAGGA-3′ |
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