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The Scientific World Journal
Volume 2012 (2012), Article ID 723293, 6 pages
http://dx.doi.org/10.1100/2012/723293
Research Article

Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh

1Department of Plant Pathology, Bangabadhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
2Nanotechnology and Catalysis Research Center, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
3Institute of Tropical Agriculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Malaysia
4Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Malaysia

Received 8 January 2012; Accepted 12 February 2012

Academic Editors: F. J. de Bruijn, J.-F. Hausman, and D. Zhou

Copyright © 2012 M. M. Rahman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

A total of 31 bacterial strains, designated as R-1 to R-31, were isolated from the rhizosphere of various plants and described in table 1. None of this strain demonstrated antagonistic activity against the soft soft rot causing bacterial strain P-138. A total of 48 bacterial strains, designated as E-35 to E-82, were extracted from the endophytes of 19 different plant species from 5 different potato-farming areas of Bangladesh and presented in table 2. Only the isolates E-45 and E-65 demonstrated antagonistic activity in in vitro test. A total of 4, 3 and 12 bacterial strains were isolated from compost fertilizer (C-28-C-31), atmosphere (A-59- A-61) and BSMRAU laboratory (L-85-L-96) and are preseted in table 3. None of these bacterial isolates demonstrated antagonistic activity against the soft soft rot causing bacterial strain P-138. The species identification schemes of isolates E-45 and E-65 using various physiological and biochemical tests are presented in tables 4-6. The combined sets of identification tests strongly suggested that antagonistic function containg isolates E-45 and E-65 were belonged to Lactobacillus and Bacillus species, respectively.

  1. Supplementary Tables