Effect of ADH II Deficiency on the Intracellular Redox Homeostasis in Zymomonas mobilis

Figure 2

Time course of the intracellular NADH (a) and NADPH (b) pools in glycolysing cell suspensions after switching from anaerobic incubation to aeration (at zero time); (▲) Zm6, (■) adhB-. Cells were grown overnight without aeration, washed, resuspended at 7 mg dry wt mL⁻¹ in 50 mM potassium phosphate buffer, pH 6.9, and gassed with oxygen-free nitrogen (2 L min⁻¹ flow through 50 mL of cell suspension). Glucose was added 10 minutes before the start of aeration, at 10 g L⁻¹ final concentration. Aerobic conditions were set by switching from oxygen-free nitrogen to gassing with air at 2 L min⁻¹ flow rate.

(a)

(b)