Identification of Proteins with Potential Osteogenic Activity Present in the Water-Soluble Matrix Proteins from Crassostrea gigas Nacre Using a Proteomic Approach

Table 1

Significant results from the mass spectrometry (LC-MS/MS) through the analysis on the MASCOT web search engine.

Spot	EST accession number	Database	Taxonomy	Mw (KDa)/pI exp.	Mw (KDa)/pI theo.	Mowse score	Range (%)	High score peptide	Peptide 𝑒-value
WSM 2	gi|164584724	EST_others	Crassostrea gigas	30/4,2	24,195/6,45	388	44%	TTVVSTHNYNQFGPR	0.00024
	gi|164584223				23,488/5,24	301	26%	DSIMDVSCVHTNSFQGSTK	1.4𝑒−07
	gi|164576479				25,192/8,52	142	14%	SIYVGDSFLDENFDIK	2.3𝑒−06
WSM 3	gi|164584724	EST_others	Crassostrea gigas	29/4,6	24,195/6,45	359	44%	TTVVSTHNYNQFGPR	0.034
	gi|164584223				23,488/5,24	236	26%	DSIMDVSCVHTNSFQGSTK	0.0036
	gi|164576479				25,192/8,52	87	7%	SIYVGDSFLDENFDIK	0.0001
WSM 4	gi|164582888	EST_others	Crassostrea gigas	28/5,2	23,109/5,28	317	28%	GFFSMSDTVHSVNCVYEAR	7.9𝑒−06
	gi|168810136				17,317/4,89	112	13%	NNGLCDYFGR	0.26
	gi|115724273	NCBInr	Strongylocentrotus purpuratus		31,155/9,14	46	3%	IEVSDLDMGR	0.037
WSM 7	gi|164582888	EST_others	Crassostrea gigas	27/5,6	23,109/5,28	258	28%	LFELEIPHR	0.055
	gi|168810136	EST_others/NCBInr			17,317/4,89	189	28%	CPPGYQGYDCGLDSSTISSSADCR	0.00086
WSM 8	gi|164570244	EST_others	Crassostrea gigas	22/6,9	19,248/8,94	192	31%	LPADDAGTGIFEPISYK	0.017
	gi|14581004		Crassostrea virginica		21,663/9,29	80	6%	TQVVAGTNYFVK	0.00078
WSM 13	gi|164570244	EST_others	Crassostrea gigas	10/5,3	19,248/8,94	258	25%	LPADDAGTGIFEPISYK	1.8𝑒−06
	gi|14581004		Crassostrea virginica		21,663/9,29	84	6%	TQVVAGTNYFVK	0.00035
WSM 14	gi|164582378	EST_others	Crassostrea gigas	25/6,2	14,929/7,59	338	57%	GICFNGISCYCPEDYIGTK	2.6𝑒−06
	gi|152813529		Crassostrea virginica		10,815/5,76	71	22%	AYRPLNFNGEMFLK	3.2
WSM 52	gi|164582378	EST_others	Crassostrea gigas	25/6,2	14,929/7,59	257	56%	GICFNGISCYCPEDYIGTK	4.8e-05