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The Scientific World Journal
Volume 2012, Article ID 834246, 6 pages
http://dx.doi.org/10.1100/2012/834246
Research Article

Effect of Aspirin on Cell Growth of Human MG-63 Osteosarcoma Line

1Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, 18071 Granada, Spain
2Institute of Neuroscience, University of Granada, 18071 Granada, Spain

Received 11 October 2011; Accepted 22 December 2011

Academic Editor: Richard D. Byrne

Copyright © 2012 E. De Luna-Bertos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used in bone tissue repair treatment for their pharmacological action. The objective of this study was to determine the effect of aspirin, on osteoblast growth, using MG63 cell line as osteoblast model. MTT spectrophotometry results showed that 20, 100, and 1000 μM aspirin doses have an inhibitory effect on growth. Cell cycle analysis revealed that aspirin doses of 100 and 1000 μM arrest the cell cycle in phase GO/G1. Parallel apoptosis/necrosis studies showed no changes in comparison to control cells after treatment with 1 or 10 μM aspirin but a significantly increased percentage of cells in apoptosis at doses of 20, 100, and 1000 μM. We highlight that treatment of osteoblast-like cells with 1000 μM aspirin increased not only the percentage of cells in apoptosis but also the percentage of necrotic cells, which was not observed in aspirin treatments at lower doses.