Research Article

Radioprotective Effect of Alk(en)yl Thiosulfates Derived from Allium Vegetables against DNA Damage Caused by X-Ray Irradiation in Cultured Cells: Antiradiation Potential of Onions and Garlic

Figure 2

The effect of alk(en)yl thiosulfates on X-ray-induced DNA damage in rat hepatoma H4IIE cells. Cells were pretreated with different concentrations (1–50 μM) of sodium n-propyl thiosulfate (NPTS), sodium 2-propenyl thiosulfate (2PTS), or 2.5 mg/mL lignan-containing flaxseed extract for 48 hours at 37°C before X-ray irradiation (10 Gy). Control cells (white column) were not exposed to any compound. The experiments were divided into nonirradiated (a) and X-ray-irradiated (b) groups. The assessment of DNA damage was carried out on 50–100 individual cells per treatment using a comet assay. Data were analyzed by a one-way factorial analysis of variance with post hoc tests (Tukey’s method). 𝑃 < 0 . 0 1 and 𝑃 < 0 . 0 0 1 versus control or between groups. ND: not determined.
846750.fig.002a
(a)
846750.fig.002b
(b)