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The Scientific World Journal
Volume 2012 (2012), Article ID 854096, 7 pages
http://dx.doi.org/10.1100/2012/854096
Research Article

Evaluation of Melanogenesis in A-375 Cells in the Presence of DMSO and Analysis of Pyrolytic Profile of Isolated Melanin

1Department of Biopharmacy, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland
2Department of Molecular Biology, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland
3Department of Instrumental Analysis, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland
4Department of Biotechnology and Genetic Engineering, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland

Received 26 October 2011; Accepted 19 December 2011

Academic Editor: Jahn M. Nesland

Copyright © 2012 Ewa Chodurek et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The increase of a skin malignant melanoma (melanoma malignum) incidence in the world has been observed in recent years. The tumour, especially in advanced stadium with metastases, is highly resistant to conventional treatment. One of the strategies is to modulate melanogenesis using chemical compounds. In this study, the processes of differentiation and melanogenesis induced by dimethylsulfoxide (DMSO) in human melanoma cells (A-375) were investigated. Natural melanin isolated from A-375 melanoma cell line treated with 0.3% DMSO was analyzed by pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) method. The products derived from pheomelanin have not been stated in the pyrolytic profile of analyzed melanin. Within all products derived from eumelanins, 1,2-benzenediol has been predominated. It has been shown that in the melanoma cells stimulated with 0.3% and 1% DMSO, the increase of transcriptional activity of the tyrosinase gene took place. It was accompanied by the rise of tyrosinase activity and an accumulation of melanin in the cells. The better knowledge about the structure of melanins can contribute to establish the uniform criteria of malignant melanoma morbidity risk.