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The Scientific World Journal
Volume 2012, Article ID 859243, 7 pages
Research Article

Nemesia Root Hair Response to Paper Pulp Substrate for Micropropagation

1Groupement de Recherche Eau, Sol, Environnement (GRESE EA4330), Laboratoire de Botanique et Cryptogamie, Faculté de Pharmacie, Université de Limoges, 2 rue du Docteur Marcland, 87025 Limoges Cedex, France
2Laboratoire de Chimie des Substances Naturelles (LCSN EA 1069), Faculté des Sciences et Techniques, Université de Limoges, 123 Avenue Albert Thomas, 87060 Limoges Cedex, France
3Laboratoire de Pharmacognosie et de Mycologie, UMR CNRS 6226 SCR, Université de Rennes 1, Equipe PNSCM, 2 Avenue du Professeur Léon Bernard, 35043 Rennes Cedex, France

Received 14 October 2011; Accepted 15 November 2011

Academic Editors: H. P. Bais and C. Gehring

Copyright © 2012 Pascal Labrousse et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Agar substrates for in vitro culture are well adapted to plant micropropagation, but not to plant rooting and acclimatization. Conversely, paper-pulp-based substrates appear as potentially well adapted for in vitro culture and functional root production. To reinforce this hypothesis, this study compares in vitro development of nemesia on several substrates. Strong differences between nemesia roots growing in agar or in paper-pulp substrates were evidenced through scanning electron microscopy. Roots developed in agar have shorter hairs, larger rhizodermal cells, and less organized root caps than those growing on paper pulp. In conclusion, it should be noted that in this study, in vitro microporous substrates such as paper pulp lead to the production of similar root hairs to those found in greenhouse peat substrates. Consequently, if agar could be used for micropropagation, rooting, and plant acclimatization, enhancement could be achieved if rooting stage was performed on micro-porous substrates such as paper pulp.