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The Scientific World Journal
Volume 2012, Article ID 901516, 9 pages
Research Article

Immunostimulant Effect of Egyptian Propolis in Rabbits

1Department of Parasitology and Animal Diseases, National Research Center, Dokki, P.O. Box 12622, Giza, Egypt
2Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt

Received 1 November 2011; Accepted 29 December 2011

Academic Editor: Halis Oguz

Copyright © 2012 Somya A. Nassar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present experiment was conducted to study the effect of ethanolic extract of Egyptian propolis given alone or in combination with inactivated Pasteurella multocida vaccine on rabbits challenged with a virulent strain of Pasteurella multocida. Fifty-six New-Zealand rabbits, 6–8 weeks old and non-vaccinated against pasteurellosis, were randomly divided into eight equal groups. The first group was kept as a control for the experiment. The other groups received different treatments with propolis extract, inactivated vaccine, or both. The experiment continued for seven weeks during which clinical signs, body weight, and mortality rate were monitored, and blood samples were collected weekly for evaluating the leukogram, serum biochemistry, and immune response in all groups of animals. At the end of the seventh week, the animals were subjected to challenge with a virulent strain of Pasteurella multocida. Two weeks later, tissue specimens were collected from different organs for histopathological examination. Results showed that rabbits of the groups treated with both propolis and the vaccine by different routes appeared healthy after challenge. It has been concluded that alcoholic extract of propolis administrated in combination with inactivated Pasteurella multocida vaccine has no adverse effects on the general health conditions and enhances immune response in rabbits.