An Optimized Real-Time PCR to Avoid Species-/Tissue-Associated Inhibition for H5N1 Detection in Ferret and Monkey Tissues
Figure 1
Agarose gel electrophoresis of SYBR Green real-time PCR products of H5N1 transcripts in lung and small intestine tissues. Lane 1: DL2000 Marker; Lane 2: positive control; Lanes 3–5: small intestine samples amplified with primer pairs N1-1/N1-2, M30F/M264R, H5-1/H5-3; Lanes 6–8: lung samples amplified with primer pairs N1-1/N1-2, M30F/M264R, H5-1/H5-3; Lanes 9–13: small intestine samples prepared by TR, QI, BI, AM, and RO, respectively, and amplified by primer pair SZNP-F2/R2; Lanes 14–18: lung samples prepared by TR, QI, BI, AM, and RO, respectively, and amplified with primer pair SZNP-F2/R2; Lane 19: negative control. (a) was for ferret tissues and (b) was for monkey tissues.
