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The Scientific World Journal
Volume 2012, Article ID 914129, 7 pages
Research Article

Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico)

1Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Severo Ochoa s/n, 18071 Granada, Spain
2Departamento de Investigación Biomédica, Facultad de Medicina, UAQ, 76050 Santiago de Queretaro, QRO, Mexico
3Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, UAM, 28049 Madrid, Spain

Received 8 November 2011; Accepted 13 December 2011

Academic Editor: Yoshihisa Hashiguchi

Copyright © 2012 Ángeles López-Céspedes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objectives. To evaluate the potential of iron-oxide dismutase excreted (SODeCRU) by T. cruzi as the antigen fraction in the serodiagnosis of Chagas disease and compile new epidemiological data on the seroprevalence of this disease in the suburban population of the city of Santiago de Querétaro (Mexico). Design and Methods. 258 human sera were analyzed by the techniques of ELISA and Western blot and using the homogenate and the SODeCRU. Results. A total of 31 sera were positive against ELISA/SODeCRU (12.4%), while 30 sera proved positive by WB/SODeCRU (11.6%). The comparison between the technique of ELISA and WB showed a sensitivity of 93%, and a specificity of 99%. The positive predictive value was 93% and the negative predictive value was 99%, with a Kappa (κ) value of 1. Conclusions. These preliminary data reveal the degree of infection of nonrural areas of Mexico and demonstrated that SODeCRU is an antigen useful to diagnose Chagas disease.