Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae)

Table 1

Matrix of central composite design (CCD) applied to evaluate the influence of reaction time and AlCl₃ concentration on the method responses (absorbance).

	Coded variables		Natural variables		Method response
Experiment	Time (min)	AlCl3 (%)	Time (min)	AlCl3 (%)	Drug (A.U.)	Extractives (A.U.)
1	−1	−1	15	2.5	0.255	0.400
2	1	−1	35	2.5	0.258	0.398
3	−1	1	15	7.5	0.258	0.409
4	1	1	35	7.5	0.264	0.400
5	0	0	25	5	0.258	0.409
6	0	0	25	5	0.258	0.410
7	0	0	25	5	0.256	0.409
8	0	−1.414	25	1.46	0.262	0.397
9	0	1.414	25	8.54	0.259	0.413
10	−1.414	0	10.86	5	0.260	0.405
11	1.414	0	39.14	5	0.260	0.414

A.U.: absorbance units.