The Scientific World Journal

Research Article

In Vitro Conservation of Twenty-Three Overexploited Medicinal Plants Belonging to the Indian Sub Continent

Table 2

Morphogenic level along with the optimised medium and glasshouse hardening status of 23 plant species processed for in vitro conservation.


S. no.	Plant species	Explant used	Callus	Cultures established at the level of
S. no.	Plant species	Explant used	Callus	Suspension	Axillary/multiple shoots	Flowering	Synseed	Rooted plants	Time taken/success rate during hardening

(1)	Acorus calamus	Node	−	−	+ 5.0KN*	−	−	+ 3.0IBA	3-4 wks/60%
(2)	Aloe vera	Basal disc	−	−	+ 5.0KN**	−	−	+ 1.0IAA	3-4 wks/90%
(3)	Bacopa moneeiri	Leaf	+ 3.0BAP; 1.0IAA	−	+ MS0***	−	+	+ MS0	2 wks/100%
(4)	Berginia ciliata	Node	−	−	+ 3.0BAP; 0.1IAA; 40AA	−	−	+ 3.0IAA	5-6 wks/30%
(5)	Coleus forskohlii	Node	+ 2.5NAA	−	+ 0.2BAP	−	−	+ 2.5IAA	3-4 wks/70%
(6)	Elaeocarpus spehericus	Leaf	+ 2.0 NAA; 0.5KN	−	−	−	−	−	−
(7)	Gentiana kurroo	Node	−	−	+ 5.0KN	−	+	+ 2.0IAA	5-6 wks/50%
(8)	Glycyrrhiza glabra	Node	+ 0.5NAA; 0.5-2,4-D; 1000CH	+ 0.5NAA; 0.5-2,4-D; 1000CH	+ 2.5KN	−	+	+ 1.0IAA	2-3 wks/80%
(9)	Indigofera tinctoria	Seeds	−	−	+ 1.0BAP	−	−	+ 0.1IAA	5-6 wks/40%
(10)	Jurinea mollis	Leaf	+ 3.0BAP; 1.0IAA)	−	−	−	−	−	−
(11)	Lavandula officinalis	Seeds	+ 0.5 NAA; 0.5-2,4-D; 0.5 KN	+ 0.5NAA; 0.5-2,4-D; 1000CH	+ 2.5KN; 0.1NAA	−	+	+ 0.5IAA	5-6 wks/50%
(12)	Ocimum sanctum	Node	−	−	+ 3.0BAP; 1.0IAA	+	−	+ 1.0IAA	3-4 wks/100%
(13)	Papaver somniferum	Seeds	+ 1.0-2,4-D; 0.1KN; 40AA	+ 2.0–2,4-D; 0.25KN	+ 0.5-2IP; 0.1 IAA	+	+	+ 1.0IAA	5-6 wks/70%
(14)	Paris polyphylla	Bulb	−	−	+ 2.0BAP	−	−	−	−
(15)	Picrorrhiza kurroa	Leaf/node	+ 1.0-2,4-D; 0.5BAP	−	+ 5.0KN	−	−	+ 3.0IBA	5-6 wks/40%
(16)	Pyrethrum cinerariefolium	Leaf/node	+ 2.0-2,4-D; 1.0KN	−	+ 5.0KN; 2.0BAP; 1.0IBA	−	−	+ 1.0IBA	5-6 wks/60%
(17)	Psoralea corylifolia	Node	−	−	+ 5.0KN; 2.0BAP; 1.0IBA	+	−	+ 1.0IBA	3-4 wks/90%
(18)	Rheum emodii	Basal disc	−		+ 3.0BAP; 1.0IAA	−	−	−	−
(19)	Salvia sclarea	Node	−	−	+ 5.0KN; 2.0BAP; 1.0IBA	−	−	+ 1.0IBA	5-6 wks/100%
(20)	Saussurea lappa	Leaf	+ 1.0-2,4-D; 0.5BAP	−	−	−	−	−	−
(21)	Swertia cordata	Leaf/node	+ 2.0-2,4-D; 1.0KN	−	+ 5.0KN; 2.0BAP; 1.0IBA	−	−	+ 3.0IBA	5-6 wks/50%
(22)	Stevia rebaudiana	Leaf/node	+ 2.0-2,4-D; 0.25KN	−	+ 0.2BAP	−	+	+ 0.1IAA	4-5 wks/100%
(23)	Valeriana wallichii	Node	−	−	+ 2.5KN	−	+	+ MS0	3-4wks/100%

*All the medium recipes were formulated using Murashige and Skoog [17] basal medium; **concentration of all the used hormone is expressed in mg/I; ***MS0: MS basal without hormone supplementation.
2,4-D: 2,4-diphenoxyacetic acid; 2IP: 2,isopentenyl adenine; AA: ascorbic acid; BAP: 6-benzylamino purine; CH: casein hydrolysate; IAA: indole-3-acetic acid; IBA: indole-3-butyric acid; KN: kinetin; NAA: α naphthalene acetic acid; wks: weeks.