Research Article
Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe
Figure 1
Excitation and emission spectra. (a): Excitation spectra (b): Emission spectra. (1) Tb3+, (2) BSA, (3) HSA, (4) Tb3+-BSA, (5) Tb3+-HSA, (6) Tb3+-Dano, (7) Tb3+-Dano-BSA, (8) Tb3+-Dano-HSA, (9) Tb3+-Dano-HSA (plasma sample). Analytical conditions: [Tb3+] = 8.5 × 10−5 mol L−1, [Dano] =1.5 × 10−4 mol L−1, [BSA] = 1 × 10−6 mol L−1, [HSA] = 1 × 10−6 mol L−1, [HSA in plasma sample] = 1 × 10−6 mol L−1, pH = 7.8, λex/λem = 347 nm/545 nm.
