Review Article

General Analytical Schemes for the Characterization of Pectin-Based Edible Gelled Systems

Table 4

Typical examples of microstructural image analysis for pectin-based gels.

Sample ingredientsExperimental conditionsMicrostructural interpretationsReferences

ALMP and acid-induced sodium caseinate gelCLSM:
Leica TCS confocal laser scanning microscope,
Fluorescence mode with a 100× 1.3 N.A. oil-immersion
objective, with an argon/krypton laser, Application of rhodamine
B to identify protein, adding droplets of dye solution into caseinate+pectin
solution, adding GDL and stirring
Homogeneousness of the sample, pore sizes,
the prevention of the formation of strands and
clusters in presence of pectin, an increase in the
staining intensity of the protein strands attached to
the network without pectin, and so on
Matia-Merino et al. [8]

Mixed HM/LM pectin gelTEM:
Transmission electron microscope (LEO 906E Electron
Microscopy Ltd., Cambridge, England),
sample size: 1 × 1×1 mm cubes,
sample preparation 20 h after gel preparation,
Fixation for 20 h in aldehyde solution based on citrate buffer,
2% glutaraldehyde, and 0.1% ruthenium red,
dehydration, polymerization, thin sectioning
Microgels, inhomogeneous structure,
LMP-Ca clusters surrounded by a coherent gel
network of HMP, dense LMP-rich areas,
sparse HMP-rich areas, aggregations, and so on
Löfgren and Hermansson [21]

Mixed ALMP and carrageenan gelCLSM:
Leica TSP2 CLSM (Leica, Mannheim, Germany) with an
argon/krypton and a helium/neon laser, fitted with a HCX PL
APO 40 × numerical aperture 1.2 oil immersion objective,
sample size: 20 ×12 × 4 mm, staining the pectin with the
antibody, localization of pectin by making cytofluorogram or by
the multivariate image feature extraction method
Heterogeneous structure, distinguishing three types
of mixed gels resulted from multi-gelling agent
formulation: interpenetrating, coupled and phase-
separated networks, and so on
Arltoft et al. [2]

HMP: high methoxyl pectin; LMP: low methoxyl pectin; ALMP: amidated low methoxyl pectin; CLSM: Confocal Laser Scanning Microscopy; TEM: Transmission Electron microscopy.