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The Scientific World Journal
Volume 2012, Article ID 969767, 6 pages
Review Article

Integration of Kinetic Analysis of Reaction Curve with a Proper Classical Approach for Enzymatic Analysis

1Unit for Analytical Probe and Protein Biotechnology, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
2College of Pharmacy and Bioengineering, Chongqing University of Science and Technology, Chongqing 400054, China
3Department of Clinical Laboratory, The Third People's Hospital of Chongqing, Chongqing 400014, China

Received 9 December 2011; Accepted 31 January 2012

Academic Editor: Salvatore Cuzzocrea

Copyright © 2012 Xiaolan Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


For enzymatic analysis to quantify a substrate or enzyme, kinetic analysis of reaction curve can be integrated with a proper classical approach. For their integration, they should have consistent slopes and intercepts of linear response and an overlapped region of analyte quantities measurable under optimized conditions. To quantify a substrate after optimizations of tool enzyme activity and reaction duration, the equilibrium method works when the reaction is completed within the reaction duration; otherwise, kinetic analysis of reaction curve applies providing at least seven data with sufficient consumption of substrate. To quantify an enzyme after optimizations of initial substrate concentration and reaction duration, the classical initial rate method works when an estimated initial rate locates within the linear range; otherwise, kinetic analysis of reaction curve applies after the conversion of the quantification index with optimized parameters. This integration strategy has ideal linear ranges and practical efficiency for quantifying an enzyme at moderate substrate levels and for quantifying a substrate at moderate cost on tool enzyme; it has promise to simultaneous assays of multiple enzymes in one reaction vessel each time and ,thus, potential applications to concurrently quantify multiple serum enzymes, screen inhibitors against multiple enzyme targets, and detect multiple serum components by enzymeimmunoassay.