Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm Formation

Figure 5

Analysis of eDNA present in various preparations used for biofilm augmentation. Agarose gel (0.8%) showing Lane 1: Luria broth control, Lane 2: purified eDNA, Lane 3: lysed membrane vesicle suspension, Lane 4: cell-free supernatant (CFS), Lane 5: concentrated cell-free supernatant (15× CFS), and Lane 6: whole cell lysate. DNA can be seen well separated on agarose gel (Lanes 2,3: purified eDNA and DNA from membrane vesicles); however, in case of CFS, 15× CFS, and whole cell lysate (lane 4, 5, 6.), it can be seen in the wells.