Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells
Figure 2
Nucleotide sequence of the 5′-flanking region of the FUT1 gene and promoter activities of the FUT1 reporter gene in DLD-1 cells. (a) Consensus transcription-factor-binding motifs are detectable in the 5′-flanking region of FUT1. Nucleotides are numbered relative to the transcription start site obtained from 5′-RACE analysis (i.e., the observed transcription start site was set to +1). (b) FUT1 promoter deletion constructs and their luciferase activities in DLD-1 cells. Luciferase reported plasmids were constructed from DLD-1 genomic DNA by PCR and ligation. In each case, each plasmid construct and pRL-CMV were cotransfected into DLD-1 cells and luciferase activity was determined in a dual-luciferase assay 24 h aftertransfection. Firefly luciferase activities were normalized to Renilla luciferase activity to correct for differences in transfection efficiency. The results obtained in three independent experiments are expressed as mean ± SD. Significant differences () are indicated by asterisks.
