The Scientific World Journal

Research Article

Evaluation of Antioxidant, Immunomodulatory, and Cytotoxic Action of Fractions from Eugenia uniflora L. and Eugenia malaccensis L.: Correlation with Polyphenol and Flavanoid Content

Table 2

Cytotoxicity assay using splenocytes from BALB/c mice treated with extracts of E. uniflora and E. malaccensis.
ExtractConcentrations (μg/mL)
15102550100
E. uniflora       
 LEtOAc Fr.−−−−++
 Ln-BuOH Fr.−−−−−+
 LH2O Fr.−−−−−−
 SEtOAc Fr.−−−+++
 Sn-BuOH Fr.−−−−−+
 SH2O Fr.−−−−−−
E. malaccensis       
 LEtOAc Fr.−−−−++
 Ln-BuOH Fr.−−−−++
 LH2O Fr.−−−−−+
 SEtOAc Fr.−−−+++
 Sn-BuOH Fr.−−−−−+
 SH2O Fr.−−−−−−
Saponin**++++++
Legend: *Nontoxic concentrations were determined by 3H-thymidine incorporation. Percentages below 30% compared to the immune cells not treated (control) are considered nontoxic (−). (LEtOAc Fr.): Ethyl acetate fraction of leaves, (Ln-BuOH Fr.): butanol fraction of leaves, (LH2O Fr.): aqueous fraction of leaves, (SEtOAc Fr.): ethyl acetate fraction of seeds, (Sn-BuOH Fr.): butanol fraction of seeds, and (SH2O Fr.): aqueous fraction of seeds.