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The Scientific World Journal
Volume 2013 (2013), Article ID 148727, 5 pages
http://dx.doi.org/10.1155/2013/148727
Clinical Study

Idiopathic Granulomatous Mastitis: An Autoimmune Disease?

1Department of General Surgery, Sakarya University Faculty of Medicine, Sakarya, Turkey
2Department of Microbiology, Sakarya University Faculty of Medicine, Sakarya, Turkey
3Department of General Surgery, Sakarya University Research and Educational Hospital, Sakarya, Turkey

Received 3 June 2013; Accepted 3 August 2013

Academic Editors: E. de Bree and M. De Luca

Copyright © 2013 Fatih Altintoprak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. This study aimed to investigate the autoimmune basis of idiopathic granulomatous mastitis (IGM) by determining the anti-nuclear antibody (ANA) and extractable nuclear antigen (ENA) levels of patients diagnosed with IGM. Material and Methods. Twenty-six IGM patients were evaluated. Serum samples were analyzed for autoantibodies by indirect immunofluorescence (IIF) using a substrate kit that induced fluorescein-conjugated goat antibodies to human immunoglobulin G (IgG). IIF patterns were read at serum dilutions of 1 : 40 and 1 : 100 for ANA positivity. Using the immunoblot technique, the sera of patients were assayed at dilutions of 1 : 40 and 1 : 100 for human autoantibodies of the IgG class to 15 lines of highly purified ENAs. Results. In the IIF studies for ANA, positivity was identified for four different patterns in the 1 : 40 diluted preparations, for three different patients in the 1 : 100 diluted preparations and only one pattern was identified at the 1 : 320 dilution. In the ENA studies, positivity was identified for four different pattern in the 1 : 40 dilution, and only one pattern was identified at the 1 : 100 dilution. Conclusion. This study was not able to support the eventual existence of an autoimmune basis for IGM.