Genetic Structure and Expression of the Surface Glycoprotein GP82, the Main Adhesin of Trypanosoma cruzi Metacyclic Trypomastigotes
Figure 2
The modular architecture of GP82 family. (a) Structure of GP82 core proteins deduced from cDNA and genomic sequences. Sequences from cDNA clones are listed in the legend of Figure 1. SL (ABR19836) is a truncated cDNA sequence obtained by RT-PCR. The slashes indicate that sequence is interrupted. For ease of viewing, the putative C-terminal was drawn in the same line. GenBank accession numbers are in parentheses. The genomic sequences (GeneBank: XP_811683, XP_810614, XP_811916) were from the T. cruzi genome sequencing project (clone CL Brener). Potential initiator methionines (M), predicted N-amino terminal signal peptide (SP), and potential GPI-anchor sequence are indicated in red, gray, and black, respectively. Not drawn to scale. (b) Alignment of GP82 sequences showing the variation of N-terminal region. Potential initiator methionines (M) are indicated in red and the predicted N-amino terminal signal peptide (PS) is boxed. cDNA sequences: ABR19835, clones 4, 9, 16, IK10 and 27, ABR19835, ABR19836; genomic sequences were extracted from the T. cruzi genome sequencing project (clone CL Brener) and are indicated by the prefix XP_.
