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The Scientific World Journal
Volume 2013 (2013), Article ID 168376, 10 pages
http://dx.doi.org/10.1155/2013/168376
Research Article

Alterations in Red Blood Cells and Plasma Properties after Acute Single Bout of Exercise

1Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland
2Department of Thermobiology, University of Lodz, 90-236 Lodz, Poland
3Department of Sport Medicine, Medical University of Lodz, 90-647 Lodz, Poland

Received 17 September 2013; Accepted 12 November 2013

Academic Editors: H. Grant, K. Kiselyov, and L. A. Videla

Copyright © 2013 Krzysztof Gwozdzinski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to investigate alterations in haemoglobin conformation and parameters related to oxidative stress in whole erythrocytes, membranes, and plasma after a single bout of exercise in a group of young untrained men. Venous blood samples from eleven healthy young untrained males (age = 22 ± 2 years, BMI = 23 ± 2.5 kg/m2) were taken from the antecubital vein before an incremental cycling exercise test, immediately after exercise, and 1 hour after exercise. Individual heart rate response to this exercise was 195 ± 12 beats/min and the maximum wattage was 292 ± 27 W. Immediately after exercise, significant increase in standard parameters (haemoglobin, haematocrit, lactate levels, and plasma volume) of blood was observed as well as plasma antioxidant capacity one hour after exercise. Reversible conformational changes in haemoglobin, measured using a maleimide spin label, were found immediately following exercise. The concentration of ascorbic acid inside erythrocytes significantly decreased after exercise. A significant decline in membrane thiols was observed one hour after exercise, but simultaneously an increase in plasma thiols immediately after and 1 h after exercise was also observed. This study shows that a single bout of exercise can lead to mobilization of defensive antioxidant systems in blood against oxidative stress in young untrained men.