Essential Roles of PKA, iNOS, CD95/CD95L, and Terminal Caspases in Suppression of Eosinopoiesis by PGE2 and Other cAMP-Elevating Agents
Isoproterenol and PGE2 share intracellular signaling mechanisms. Bone-marrow cultures were established in the presence of IL-5, alone or associated with adenylate cyclase inhibitor SQ22536 (a), PKA selective inhibitor H89 (b), PKC/PKA dual inhibitor Bis (c), or the indicated combinations of these agents, which were all present from the beginning of culture for a 7-day period. Data (mean + SEM; ) are the numbers of EPO+ cells recovered at the end of the culture (a) or the % eosinophilopoiesis suppression in the indicated conditions, where inhibitors were used to prevent this suppression ((b) and (c)). Significant differences relative to the respective IL-5 controls: , , , .
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