Essential Roles of PKA, iNOS, CD95/CD95L, and Terminal Caspases in Suppression of Eosinopoiesis by PGE2 and Other cAMP-Elevating Agents
Impact of CD95L and CD95 deficiency in bone-marrow responses to cAMP-dependent agonists and to an NO donor. Bone-marrow cultures were established from BALB/c and mutant gld mice of the same background, lacking CD95L ((a) and (b)) or from C57BL/6 and mutant lpr mice of the same background, lacking CD95 ((c) and (d)), in the presence of IL-5, alone or associated with the indicated concentrations of PGE2, isoproterenol (Iso), cholera toxin (Ch T), Rolipram (Rol), and dibutyryl cAMP (dbcAMP). Where indicated, sodium nitroprusside (SNP), an NO-releasing chemical, was used to evaluate the effectiveness of NO. All agents were present from the beginning of culture for a 7-day period. Data (mean + SEM; in (c), remaining panels) are the numbers of EPO+ cells recovered at the end of the culture. Significant differences relative to the respective IL-5 controls: , , , .
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