The Antinociceptive and Anti-Inflammatory Activities of Aspidosperma tomentosum (Apocynaceae)
Table 4
Effects of extract, fractions (100 mg/kg, p.o.), isorhamnetin, and indomethacin (100 μmol/kg, p.o.) in nociception induced by formalin.
Group
Linking (s)
% of inhibition
Phase 1
Phase 2
Phase 1
Phase 2
Mean ± S.E.M.
Mean ± S.E.M.
Mean ± S.E.M.
Mean ± S.E.M.
Vehicle
6
82.8 ± 5.6
215.7 ± 24.0
—
—
Indomethacin
6
59.4 ± 8.1
94.6 ± 24.4
23.4*
56.1**
CEE
6
39.6 ± 2.4
75.8 ± 29.7
55.0**
64.8**
Isorhamnetin
6
59.4 ± 6.3
55.6 ± 32.7
35.7**
74.2**
Soluble phase
6
41.8 ± 3.4
139.6 ± 10.5
52.3**
35.3
Hexane 100%
6
59.2 ± 4.4
195.0 ± 28.2
31.8**
9.6
Hex : CHCl3 50%
6
62.7 ± 3.9
204.5 ± 42.4
24.2**
5.2
CHCl3 100%
6
35.5 ± 7.0
51.2 ± 19.3
57.1**
76.2**
CHCl3 : EtAcO 50%
6
46.0 ± 5.2
131.2 ± 18.4
49.6**
39.2
EtAcO 100%
6
56.0 ± 2.8
128.8 ± 9.7
32.4**
40.3
CHCl3 : MeOH 5%
6
59.6 ± 3.7
84.6 ± 27.7
31.8**
60.8**
CHCl3 : MeOH 10%
6
42.2 ± 2.6
177.8 ± 16.3
52.0**
17.6
Statistical differences between the treated and the control groups were evaluated by ANOVA and Dunnett test, and the asterisks denote the significance levels in comparison with control groups: *; **.
