Figure 1: HCF immunization protects against CT26 tumor growth. (a) BALB/cJ mice ( ) were vaccinated three times in two-week intervals with human HCF in alum before CT26 cells challenge. (b) Control mice ( ) were treated with PBS in alum. Tumor growth was measured regularly using a caliper. Tumor volume (mm3) = (4/3) × pi × R 1 × R 2 × R 3. Tumor sizes were significantly lower in mice immunized with HCF as compared to the control group ( ).