Figure 5: Proteomic analysis of CT26 antigens identified by anti-HCF antibodies. 2DE was performed with 200 μg protein using strips of 7 cm nonlinear pH range of 3–11 and 12.5% SDS-PAGE. (a) Gel was stained with Coomassie Brilliant Blue. (b) Western blot using anti-HCF serum. (c) Western blot using preimmune serum. By mass spectroscopy, spot 1 was identified as mortalin, whereas spot 3 was identified as creatine kinase M-type. Spots 2, 4, and 5 were not identified using the tool online of MASCOT program (http://www.matrixscience.com/).