Anti-Tumor Effect of Rutin on Human Neuroblastoma Cell Lines through Inducing G2/M Cell Cycle Arrest and Promoting Apoptosis
Figure 4
The effect of rutin on cell apoptosis and expression of BCL2 and BAX. (a) Apoptosis rates of LAN-5 incubated with different concentrations of rutin, the percentage of apoptotic cells were measured by flow cytometry using the PI-annexin V assay. (b) Quantification of BCL2 and BAX mRNA levels, (c) calculated BCL2/BAX ratios. The “relative intensity” was defined as the intensity (target mRNA)/intensity (GAPDH). All experiments were repeated three times. , compared to control. 1: marker; 2: control; 3: RT 25 μM; 4: RT 50 μM; 5: Rt 100 μM.
