The Scientific World Journal / 2013 / Article / Tab 6

Research Article

Evaluation of Genotoxic Effects of New Molecules with Possible Trypanocidal Activity for Chagas Disease Treatment

Table 6

PTAL 04-09 induction of mutagenicity and cytotoxicity in Salmonella enterica serovar Typhimurium standard strains, without (−S9) and with (+S9) metabolic activation.

StrainsDose ( g/mL)−S9+S9
Mean ± S.D. (M.I.a)SurvivalbMean ± S.D. (M.I.a)Survivalb

TA97087 ± 9.8 (1.00) 10097 ± 25 (1.00)100
1.0 85 ± 95 (0.98)9774 ± 13 (0.76)77
5.0100 ± 5.7 (1.15)10080 ± 13 (0.82)73
10115 ± 16 (1.33)10078 ± 16 (0.80)77
50143 ± 11 (1.65)10082 ± 9.6 (0.85)77

TA98045 ± 1.5 (1.00) 10018 ± 6.4 (1.00)100
1.0 66 ± 11 (1.46)9318 ± 8.5 (0.98)100
5.064 ± 3.0 (1.40)10017 ± 2.8 (0.91)100
1065 ± 5.1 (1.43)10015 ± 1.5 (0.82)71
5066 ± 7.0 (1.45)10016 ± 2.0 (0.88)62

TA1000120 ± 23 (1.00)100117 ± 19 (1.00)100
1.0 113 ± 12 (0.94)100129 ± 18 (1.11)66
5.0165 ± 12 (1.37)100144 ± 14 (1.23)59
10192 ± 9.4 (1.59)100169 ± 9.4 (1.45)60
50439 ± 39 (3.64*)100297 ± 12 (2.53*)55

TA1535022 ± 3.2 (1.00)1005.3 ± 1.5 (1.00)100
1.0 21 ± 1.5 (0.94)977.3 ± 1.1 (1.38)41
5.024 ± 2.0 (1.09)958.3 ± 2.3 (1.56)41
1025 ± 4.5 (1.10)1008.6 ± 1.1 (1.63)39
5026 ± 1.5 (1.18)935.6 ± 1.1 (1.06)36

TA1020254 ± 16 (1.00)100265 ± 12 (1.00)100
1.0 258 ± 19 (1.02)100266 ± 24 (1.01)100
5.0262 ± 24 (1.03)100248 ± 9.1 (0.94)100
10262 ± 31 (1.03)100260 ± 30 (0.98)100
50274 ± 16 (1.08)100265 ± 7.6 (1.00)100

Mutagenic index (M.I.): ratio of the his + induced with samples/spontaneous his + in the negative control. S.D.: standard deviation. Positive responses marked in bold when M.I. ≥ 2.
bPercent of cellular survival: <70% was considered toxic dose and it is marked in bold.
*Representation of a significant response ( ).
Negative control was DMSO. Positive controls and their M.I., with and without S9 mix, respectively, were: 4NQO (7.19) and 2AA (9.55) for TA97, 4NQO (10) and 2AA (3.95) for TA98; AS (13) and 2AA (2.10) for TA100, AS (18) and 2AA (6.25) for TA1535, and MitC (9.28 and 6.86) for TA102.

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