The Scientific World Journal / 2013 / Article / Fig 3

Research Article

Association of Self-DNA Mediated TLR9-Related Gene, DNA Methyltransferase, and Cytokeratin Protein Expression Alterations in HT29-Cells to DNA Fragment Length and Methylation Status

Figure 3

Distribution of cytokeratine (CK; (a)–(c)) and DNA methyltransferase 3A (DNMT3A; (d)–(f)) immunoreactive HT29 cells. (a) and (d) -axes represent the intensities of immunoreactions from negative (−) to strong (+++). -axes represent the distribution (percentage) of cells showing different immunoreaction intensities (blue columns: control cells; red columns: type 3. DNA-treated cells). (b) and (c) CK expression before (b) and after (c) type 3. DNA incubation (immunoreactive cells/arrows/display brownish cytoplasmic reaction, 200x magnification, hematoxylin costaining). (e) and (f) DNMT3A expression before (e) and after (f) type 3. DNA incubation (immunoreactive cells/arrows/displayed brownish cytoplasmic reaction, 200x magnification, hematoxylin costaining).
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