Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein
Effect of isoimperatorin on the quenching of HSA-warfarin complex (a) and HSA-ibuprofen complex (b) fluorescence. From 1 to 8: [HSA] = [warfarin or iboprufen] = 3 μM, [isoimperatorin] = 0, 0.4, 0.8, 1.2, 1.6, 2, 2.5, and 3 μM. All reproduced data shown are representative of at least 3 independent experiments. (c) Fluorescence quenching profiles of HSA (◆), HSA-ibobrofen (■), and HSA-warfarin (▲) in the presence of various concentration of isoimperatorin.