Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein

Table 3

Surface hydrophobicity parameters for HSA in the absence and the presence of isoimperatorin.

Parameters	HSA	HSA-IIM complex
𝐾^app𝑑 (𝜇M)	0.9±0.05^∗	1.4±0.07^∗
𝐹max/[HSA]	1357.14±8.5^∗∗	1128.53±6.3^∗∗
Surface hydrophobicity index (𝐹max/[HSA] 𝐾^app𝑑)	1507.94±11.46^∗∗	806.12±0.07^∗∗

Data are expressed as mean ± SD of three measurements. *𝑃value<0.0006, **𝑃value<0.0001.