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The Scientific World Journal
Volume 2013, Article ID 362453, 5 pages
http://dx.doi.org/10.1155/2013/362453
Research Article

Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence Detection

Department of Pharmacology and Toxicology, National Veterinary Research Institute, 57 Partyzantow Avenue, 24-100 Pulawy, Poland

Received 27 August 2013; Accepted 30 September 2013

Academic Editors: H. Yang and R. A. Yokley

Copyright © 2013 Konrad Pietruk and Piotr Jedziniak. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A labour- and time-effective analytical procedure for determination of ivermectin in medicated feed at recommended level of 2.0 mg kg−1 has been developed and validated. The analyte was extracted from grinded feed samples with acetonitrile and derivatisated with N-methylimidazole and trifluoracetic anhydride. The fluorescent derivatives were analysed by liquid chromatography method using C8 column. The isocratic conditions using acetonitrile, methanol, water, and tetrahydrofuran were applied. Fluorescence detection was performed at 365 nm (excitation) and 475 nm (emission) wavelengths. The total analysis time was 10 min. The validation results of the method (within-laboratory reproducibility 4.0% CV, mean recovery 100.1%) confirm the appropriate precision and accuracy of the developed method.