Determination of Ivermectin in Medicated Feeds by Liquid Chromatography with Fluorescence Detection

Table 1

Validation results.

	Day 1	Day 2	Day 3
Standard calibration curve	𝑦=0.50𝑥−0.03, 𝑅^2=0.99
Matrix calibration curve	𝑦=0.48𝑥+0.03, 𝑅^2=0.99
Mean concentration (mg kg^−1); (repeatability)	2.00	1.97	2.04
Standard deviation (mg kg^−1); (repeatability)	0.06
CV (%); (repeatability)	3.0
HorRat (repeatability)	0.38
Mean recovery (%); (repeatability)	100.1	98.4	101.8
Mean concentration (mg kg^−1); (within-laboratory reproducibility)	2.00
Standard deviation (mg kg^−1); (within-laboratory reproducibility)	0.08
CV (%); (within-laboratory reproducibility)	4.0
Mean recovery (%); (within-laboratory reproducibility)	100.1
LOD (mg kg^−1)	0.03
LOQ (mg kg^−1)	0.50
𝑃 value (ANOVA)	0.20