Table of Contents Author Guidelines Submit a Manuscript
The Scientific World Journal
Volume 2013, Article ID 364730, 7 pages
http://dx.doi.org/10.1155/2013/364730
Research Article

Conformation and Catalytic Properties Studies of Candida rugosa Lip7 via Enantioselective Esterification of Ibuprofen in Organic Solvents and Ionic Liquids

Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China

Received 30 September 2013; Accepted 23 October 2013

Academic Editors: A. A. Iglesias and A. Surguchov

Copyright © 2013 Xiang Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. V. Iyer and L. Ananthanarayan, “Enzyme stability and stabilization-aqueous and non-aqueous environment,” Process Biochemistry, vol. 43, no. 10, pp. 1019–1032, 2008. View at Publisher · View at Google Scholar · View at Scopus
  2. A. Klibanov, “Why are enzymes less active in organic solvents than in water?” Trends in Biotechnology, vol. 15, no. 3, pp. 97–101, 1997. View at Publisher · View at Google Scholar · View at Scopus
  3. B. Hernández-Rodríguez, J. Córdova, E. Bárzana, and E. Favela-Torres, “Effects of organic solvents on activity and stability of lipases produced by thermotolerant fungi in solid-state fermentation,” Journal of Molecular Catalysis B, vol. 61, no. 3-4, pp. 136–142, 2009. View at Google Scholar
  4. A. M. Klibanov, “Improving enzymes by using them in organic solvents,” Nature, vol. 409, no. 6817, pp. 241–246, 2001. View at Publisher · View at Google Scholar · View at Scopus
  5. Y. Liu, D. Chen Y Yan, and Y. Yan, “Effect of ionic liquids, organic solvents and supercritical CO2 pretreatment on the conformation and catalytic properties of Candida rugosa lipase,” Journal of Molecular Catalysis B, vol. 90, pp. 123–127, 2013. View at Google Scholar
  6. L. Xu, X. Jiang, J. Yang, Y. Liu, and Y. Yan, “Cloning of a novel lipase gene, lipJ08, from Candida rugosa and expression in Pichia pastoris by codon optimization,” Biotechnology Letters, vol. 32, no. 2, pp. 269–276, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. D. Kahveci and X. Xu, “Repeated hydrolysis process is effective for enrichment of omega 3 polyunsaturated fatty acids in salmon oil by Candida rugosa lipase,” Food Chemistry, vol. 129, no. 4, pp. 1552–1558, 2011. View at Publisher · View at Google Scholar · View at Scopus
  8. M.-M. Zheng, Y. Lu, L. Dong et al., “Immobilization of Candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters,” Bioresource Technology, vol. 115, pp. 141–146, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. J. H. Lee, S. B. Kim, S. W. Kang et al., “Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process,” Bioresource Technology, vol. 102, no. 2, pp. 2105–2108, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. T. Siódmiak, M. Ziegler-Borowska, and M. P. Marszałł, “Lipase-immobilized magnetic chitosan nanoparticles for kinetic resolution of (R,S)-ibuprofen,” Journal of Molecular Catalysis B, vol. 94, pp. 7–14, 2013. View at Google Scholar
  11. A. Ghanem, M. N. Aboul-Enein, A. El-Azzouny, and M. F. El-Behairy, “Lipase-mediated enantioselective kinetic resolution of racemic acidic drugs in non-standard organic solvents: direct chiral liquid chromatography monitoring and accurate determination of the enantiomeric excesses,” Journal of Chromatography A, vol. 1217, no. 7, pp. 1063–1074, 2010. View at Publisher · View at Google Scholar · View at Scopus
  12. G. Duan, C. B. Ching, E. Lim, and C. H. Ang, “Kinetic study of enantioselective esterification of ketoprofen with n-propanol catalysed by an lipase in an organic medium,” Biotechnology Letters, vol. 19, no. 11, pp. 1051–1055, 1997. View at Publisher · View at Google Scholar · View at Scopus
  13. Y. Liu, F. Wang, and T. Tan, “Effects of alcohol and solvent on the performance of lipase from Candida sp. in enantioselective esterification of racemic ibuprofen,” Journal of Molecular Catalysis B, vol. 56, no. 2-3, pp. 126–130, 2009. View at Publisher · View at Google Scholar · View at Scopus
  14. C.-S. Chen, Y. Fujimoto, G. Girdaukas, and C. J. Sih, “Quantitative analyses of biochemical kinetic resolutions of enantiomers,” Journal of the American Chemical Society, vol. 104, no. 25, pp. 7294–7299, 1982. View at Google Scholar · View at Scopus
  15. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Google Scholar · View at Scopus
  16. M. Carbonaro and A. Nucara, “Secondary structure of food proteins by Fourier transform spectroscopy in the mid-infrared region,” Amino Acids, vol. 38, no. 3, pp. 679–690, 2010. View at Publisher · View at Google Scholar · View at Scopus
  17. R. Pribic, I. H. M. Vanstokkum, D. Chapman, P. I. Haris, and M. Bloemendal, “Protein secondary structure from Fourier transform infrared and/or circular dichroism spectra,” Analytical Biochemistry, vol. 214, no. 2, pp. 366–378, 1993. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Yang, F. Wang, D. Lan, C. Whiteley, B. Yang, and Y. Wang, “Effects of organic solvents on activity and conformation of recombinant Candida antarctica lipase A produced by Pichia pastoris,” Process Biochemistry, vol. 47, no. 3, pp. 533–537, 2012. View at Publisher · View at Google Scholar · View at Scopus
  19. Y. Liu, H. Tan, X. Zhang, Y. Yan, and B. H. Hameed, “Effect of monohydric alcohols on enzymatic transesterification for biodiesel production,” Chemical Engineering Journal, vol. 157, no. 1, pp. 223–229, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. X. Li, T. Liu, L. Xu, X. Gui, F. Su, and Y. Yan, “Resolution of racemic ketoprofen in organic solvents by lipase from Burkholderia cepacia G63,” Biotechnology and Bioprocess Engineering, vol. 17, no. 6, pp. 1147–1155, 2012. View at Google Scholar
  21. C. Laane, S. Boeren, K. Vos, and C. Veeger, “Rules for optimization of biocatalysis in organic solvents,” Biotechnology and Bioengineering, vol. 102, no. 1, pp. 1–8, 2009. View at Google Scholar · View at Scopus
  22. D. Herbst, S. Peper, and B. Niemeyer, “Enzyme catalysis in organic solvents: influence of water content, solvent composition and temperature on Candida rugosa lipase catalyzed transesterification,” Journal of Biotechnology, vol. 162, no. 4, pp. 398–403, 2012. View at Publisher · View at Google Scholar · View at Scopus
  23. S. Pan, X. Liu, Y. Xie et al., “Esterification activity and conformation studies of Burkholderia cepacia lipase in conventional organic solvents, ionic liquids and their co-solvent mixture media,” Bioresource Technology, vol. 101, no. 24, pp. 9822–9824, 2010. View at Publisher · View at Google Scholar · View at Scopus
  24. Y. Liu, D. Chen, and Y. Yan, “Effect of ionic liquids, organic solvents and supercritical CO2 pretreatment on the conformation and catalytic properties of Candida rugosa lipase,” Journal of Molecular Catalysis B, vol. 90, pp. 123–127, 2013. View at Google Scholar
  25. J. Zheng, L. Xu, Y. Liu, X. Zhang, and Y. Yan, “Lipase-coated K2SO4 micro-crystals: preparation, characterization, and application in biodiesel production using various oil feedstocks,” Bioresource Technology, vol. 110, pp. 224–231, 2012. View at Publisher · View at Google Scholar · View at Scopus
  26. Y. Gu and G. Li, “Ionic liquids-based catalysis with solids: state of the art,” Advanced Synthesis and Catalysis, vol. 351, no. 6, pp. 817–847, 2009. View at Publisher · View at Google Scholar · View at Scopus
  27. P. Domínguez de María, J. M. . Sánchez-Montero, J. V. . Sinisterra, and A. R. . Alcántara, “Understanding Candida rugosa lipases: an overview,” Biotechnology Advances, vol. 24, no. 2, pp. 180–196, 2006. View at Google Scholar
  28. D. Chávez-Flores and J. M. Salvador, “Commercially viable resolution of ibuprofen,” Biotechnology Journal, vol. 4, no. 8, pp. 1222–1224, 2009. View at Publisher · View at Google Scholar · View at Scopus