Conformation and Catalytic Properties Studies of Candida rugosa Lip7 via Enantioselective Esterification of Ibuprofen in Organic Solvents and Ionic Liquids

Table 1

Effect of alcohols on the enzymatic esterification of ibuprofen*.

	Acyl donor	Enzyme activity (U/g)	ees (%)
1	Methanol	41.05±0.65	24.11±0.06
2	Ethanol	44.16±1.20	12.12±0.03
3	1-Propanol	33.05±1.57	16.22±0.08
4	1-Butanol	84.50±1.12	53.42±0.06
5	1-Pentanol	95.87±0.75	66.41±0.11
6	1-Hexanol	88.11±0.93	54.50±0.12
7	1-Heptanol	91.29±0.58	61.01±0.12
8	1-Octanol	91.43±0.56	69.42±0.07
9	1-Nonanol	103.9±3.91	73.91±0.05
10	1-Decanol	106.8±2.56	83.51±0.03
11	Isobutanol	93.61±2.56	74.91±0.05
12	Isoamylol	108.95±2.56	87.51±0.06
13	Isooctanol	115.46±3.12	95.72±0.04
14	tert-Butanol	ND	ND
15	tert-Amyl alcohol	ND	ND
16	1,2-Ethanediol	ND	ND

∗The reactions were performed at 50°C, 200 rpm for 12 h. 0.1 g CRL7 was added to 5 mL isooctane containing 0.1 mmol ibuprofen, 1.0 mmol alcohol (from C1 to C10). The data were measured in triplicate and expressed in mean ± standard deviation (SD); ND indicates not determined.