Conformation and Catalytic Properties Studies of Candida rugosa Lip7 via Enantioselective Esterification of Ibuprofen in Organic Solvents and Ionic Liquids

Table 3

Quantitative estimation of the secondary structure elements of the treated CRL7 calculated by FT-IR spectroscopy measurement*.

Solvent	𝛼-Helix (%)	𝛽-Sheet (%)	𝛽-Turn (%)	Random coil (%)
Control	43.46±0.02	26.91±0.02	11.83±0.03	17.79±0.04
n-Undecane	33.39±0.02	27.10±0.09	21.57±0.02	17.94±0.02
n-Decane	21.38±0.21	46.04±0.11	11.93±0.06	20.65±0.15
n-Nonane	21.03±0.03	46.43±0.03	12.76±0.13	19.78±0.07
Isooctane	39.03±0.12	37.61±0.04	13.53±0.06	18.72±0.05
n-Octane	23.62±0.06	40.95±0.16	13.75±0.12	21.68±0.08
n-Heptane	22.23±0.13	44.55±0.15	13.04±0.14	20.18±0.06
n-Hexane	38.53±0.09	27.83±0.13	13.81±0.09	19.83±0.10
Cyclohexane	21.17±0.12	45.74±0.12	12.82±0.04	20.26±0.10
Xylene	37.43±0.05	22.22±0.12	14.51±0.10	25.83±0.15
Acetonitrile	40.88±0.12	24.94±0.04	14.57±0.13	19.59±0.02
BmimTF2N	24.27±0.05	25.62±0.10	21.58±0.11	28.52±0.11
BmimPF6	24.90±0.04	14.39±0.09	19.43±0.12	41.28±0.14
EmimPF6	32.33±0.10	23.09±0.08	16.81±0.13	27.77±0.12

∗The lipase without treatment of organic solvent and ionic liquids was set as control.
The data were measured in triplicate and expressed in mean ± standard deviation (SD).