Research Article
Extraction Optimization of Tinospora cordifolia and Assessment of the Anticancer Activity of Its Alkaloid Palmatine
Table 2
Central composite design by RSM program for optimization of extraction conditions with experimental and their corresponding predicted yields.
| | Runs | Extraction temperature
(, °C) | Extraction time
(, hours) | Extraction cycles
(, cycle) | Experimental yield (%) | Predicted yield (%) |
| | 1 | 40 (0) | 32 (0) | 12 (0) | 13.29 | 11.51 | | 2 | 50 (1) | 16 (−1) | 20 (1) | 8.16 | 8.86 | | 3 | 40 (0) | 32 (0) | 12 (0) | 10.26 | 11.51 | | 4 | 30 (−1) | 48 (1) | 20 (1) | 11.32 | 11.09 | | 5 | 23.18 (−1.68) | 32 (0) | 12 (0) | 12.89 | 13.09 | | 6 | 56.82 (1.68) | 32 (0) | 12 (0) | 9.67 | 9.94 | | 7 | 40 (0) | 5.09 (−1.68) | 12 (0) | 11.93 | 11.51 | | 8 | 40 (0) | 32 (0) | −1.45 (−1.68) | 11.36 | 11.91 | | 9 | 50 (1) | 48 (1) | 4 (−1) | 12.19 | 11.82 | | 10 | 40 (0) | 32 (0) | 25.45 (1.68) | 10.83 | 11.12 | | 11 | 30 (−1) | 48 (1) | 4 (−1) | 11.97 | 11.20 | | 12 | 40 (0) | 32 (0) | 12 (0) | 11.31 | 11.51 | | 13 | 30 (−1) | 16 (−1) | 04 (−1) | 13.67 | 14.29 | | 14 | 50 (1) | 16 (−1) | 04 (−1) | 9.53 | 9.68 | | 15 | 40 (0) | 58.91 (1.68) | 12 (0) | 9.67 | 11.52 | | 16 | 40 (0) | 32 (0) | 12 (0) | 12.73 | 11.51 | | 17 | 50 (1) | 48 (1) | 20 (1) | 12.65 | 11.96 | | 18 | 40 (0) | 32 (0) | 12 (0) | 12.42 | 11.51 | | 19 | 30 (−1) | 16 (−1) | 20 (1) | 12.92 | 13.21 |
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