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The Scientific World Journal
Volume 2013, Article ID 382350, 8 pages
Research Article

Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells

1Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaan’xi 710032, China
2Department of Otolaryngology, Shenyang 463 Hospital, Xiao He Yan Road 46, Shenyang, Liaoning 110042, China
3School of Pharmacy, Institute of Materia Medica, Fourth Military Medical University, Xi’an, Shaan’xi 710032, China

Received 17 July 2013; Accepted 25 August 2013

Academic Editors: M. Edery, S. E. Harris, R. B. Petersen, and M. Zarevucka

Copyright © 2013 Xiaofang Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The intestinal absorption and metabolism characteristics of the potentially beneficial polyphenol rutin were studied by measuring the intracellular accumulation and transport of rutin into Caco-2 cells with the sensitive and reliable analytical method of HPLC-coupled tandem mass spectrometry. Rutin and glucuronidated rutin were absorbed differently by the basolateral and apical membranes, and rutin showed differential permeability through the apical and basolateral sides. Approximately 33% of the rutin was metabolized to glucuronidated rutin, and the intracellular concentration of glucuronidated rutin was much lower than that of parent rutin. P-glycoprotein and multidrug-resistant proteins 2 and 3 were involved in the transmembrane transport and intracellular accumulation of rutin by Caco-2 cells. These results suggest that a specific transport system mediates rutin movement across the apical membrane in Caco-2 cells and that metabolic enzymes are important for this process.