The Scientific World Journal / 2013 / Article / Tab 1 / Research Article
RhoA Regulation of Cardiomyocyte Differentiation Table 1 Primers used for PCR.
PCR product Forward primer (5′-3′) Reverse primer (5′-3′) Product size (bp) Elucidation of chick RhoA gene structure Chick RhoA Intron1 GCCGGAATTC ATGGCAGCCATTCGAAA1 CTATCAGGACTATCGATTG 282 bp4 Chick RhoA Intron2 GTGGAGTTGGCTTTGTGGGATAC GCTTCATTTTGGCCAGCTCT 251 bp4 Chick RhoA Intron3 AGTGGACCCCGGAAGTGA TAAGAGAAGGCACCCGGAC 287 bp4 Amplification of putative mouse RhoA promoter PromoterLong CCCGCGGATCC GTGGCGGGCAAAGCTTGCAG2 CCCGCGGATCCGTCCGGCCTCTTCGCGCT 637 PromoterShort CCCGCGGATCC AGCAATCGTGGCTGAACTGAG2 CCCGCGGATCCGTCCGGCCTCTTCGCGCT 264 Generation of dominant mutant of mouse RhoA mRhoA N19 CGCCGCTCGAGATG GCTGCCATCAGGAAGAAACTGGTGATT GTT GGTGATGGAGCTTGTGGTAAGAATTGCTTGCTCATA3 ACGCGTCGACTCACAAGATGAGGCACCC 582 Primers used for real-time PCR GAPDH TCCTACCCCCAATGTGTCCGTC GCCCAAGATGCCCTTCAGTG 121 RhoA ORF ATTGATGTGTTTTTCCATTG CTCCCGTCTCGTGTGCTCGTCATT 151 RhoA 3′UTR GCTACCAGTATTTAGAAGCCAACCAC GCTGTTAGAGCAGTGTCAGAAGGAC 88 SRF TGCCTCAACTCGCCAGACTCTC TTCAGTGTGTCCTTGGTTTCCC 140 Cardiac
-actin GCCAACCGTGAGAAGATGACC CGCCAGAATCCAGAACAATGC 130 GATA 4 ATGCCGAGGGTGAGCCTGTATG CTTCCGTTTTCTGGTTTGAATCC 110
Incorporated EcoR1 and
BamH1 sites are underlined.
ATG translation start codon and introduced N19 mutation are shown in bold.
Expected size in absence of intron.