Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9
Figure 5
Determination of invasion property of PC3/Si and PC3/Sc cells using gelatin degradation assay. The ability of PC3 cells knockdown of MMP9 to degrade the gelatin matrix was determined by culturing PC3 cells (PC3/Sc and PC3/Si) on a FITC-conjugated gelatin matrix (green). Cells were found within the degraded matrix (a). Actin staining is shown in red (a), (c), and (d). Invadopodia are indicated by arrow heads (a). Areas of degradation of the matrix are indicated by green arrows (b). The rectangular field in (c) is enlarged in (d). Focal adhesions are indicated by arrows (d). These results represent one of several experiments performed.
