Resveratrol-Loaded Polymeric Nanoparticles: Validation of an HPLC-PDA Method to Determine the Drug Entrapment and Evaluation of Its Antioxidant Activity

Table 4

Percentage of inhibition of the radical ABTS^●+ from free and nanoencapsulated RVT in concentrations of 1, 5, 10, 20, and 25 µM for 0, 24, 48, and 72 h.

RVT concentration (µM)	Inhibition of ABTS^●+ (%)
	RVT-loaded PLA nanoparticles	RVT-loaded PLA-PEG nanoparticles	Free RVT
0 h
1	6.84 (±1.04)^a	8.95 (±1.70)^a	13.29 (±1.25)^b
5	28.56 (±9.27)^a,b	15.38 (±0.76)^a	46.33 (±2.66)^b
10	35.50 (±1.58)^a	33.23 (±5.18)^a	69.21 (±1.58)^b
20	53.35 (±3.88)^a	56.40 (±2.63)^a	95.48 (±4.61)^b
25	65.54 (±7.30)^a	64.00 (±5.42)^a	99.70 (±0.29)^b
24 h
1	5.67 (±0.45)^a	2.92 (±2.79)^a	7.35 (±2.27)^a
5	10.89 (±0.58)^a	14.97 (±3.87)^a	28.68 (±3.29)^b
10	22.75 (±1.87)^a	24.09 (±1.57)^a	49.35 (±5.10)^b
20	50.49 (±4.17)^a	55.66 (±1.65)^a	95.46 (±5.20)^b
25	65.75 (±3.14)^a	75.87 (±5.86)^b	97.88 (±1.91)^c
48 h
1	2.90 (±2.33)^a,c	7.50 (±0.95)^b	2.21 (±1.98)^c
5	7.87 (±1.07)^a	21.76 (±2.17)^a	25.47 (±12.53)^a
10	20.82 (±2.30)^a	35.01 (±4.68)^b	47.74 (±4.56)^c
20	45.76 (±5.92)^a	62.14 (±320)^b	88.40 (±0.88)^c
25	69.07 (±12.22)^a	85.13 (±2.52)^a,b	98.34 (±0.68)^b
72 h
1	12.13 (±0.65)^a	14.01 (±1.07)^a	3.83 (±0.76)^b
5	13.30 (±2.19)^a	25.22 (±1.67)^b	28.30 (±1.96)^b
10	24.90 (±4.45)^a	40.58 (±4.30)^b	51.43 (±3.25)^c
20	57.26 (±13.78)^a	70.45 (±2.39)^b	92.17 (±4.86)^c
25	76.43 (±2.49)^a	90.99 (±3.45)^b	95.52 (±1.58)^b

a,b,cMean of triplicate ± standard deviation analyzed per line. Same letters mean statistical equality, and inequality stats are indicated for different letters (ANOVA with Tukey post test and α 0,05).