Resveratrol-Loaded Polymeric Nanoparticles: Validation of an HPLC-PDA Method to Determine the Drug Entrapment and Evaluation of Its Antioxidant Activity

Table 5

IC₅₀ of RVT (free or nanoencapsulated) over the capture of the ABTS^●+ cation radical in sodium phosphate buffer (50 mM, pH 7.4) and in the absence of light at room temperature (₋𝜆 = 734 nm).

Time (h)	IC50
	RVT-loaded PLA nanoparticles ± RSD*	RVT-loaded PLA-PEG nanoparticles ± RSD*	Free RVT ± RSD*
0	17.30±0.71^a	18.15±0.95^a	8.21±0.41^b
24	19.52±1.08^a	17.31±0.70^b	10.79±028^c
48	19.83±2.48^a	14.65±0.10^b	11.58±0.78^b
72	16.61±1.08^a	12.76±0.80^b	11.15±0.34^b

a,b,cMean of triplicate ± standard deviation analyzed per line. Same letters mean statistical equality and inequality stats are indicated for different letters (ANOVA with Tukey post test and α 0,05).
∗RSD: relative standard deviation.